STK40 Protein (GST Tag)

06.09.2012

Produkt-Information von STK40 Protein

Synonym	SGK495, SHIK, STK40
Protein Construction	A DNA sequence encoding the human STK40 (NP_114406) (Met1-Lys435) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von STK40 Protein

Purity	> 97 % as determined by SDS-PAGE	SDS-PAGE: STK40 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human STK40 /GST chimera consists of 672 amino acids and has a calculated molecular mass of 76.8 kDa. The recombinant protein migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of 20mM Tris,500mM NaCl,10%glycerol,3mM DTT, 0.5M Urea, 0.5mM GSH, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von STK40 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von STK40 Protein

STK40 may be a negative regulator of NF-kappa-B and p53-mediated gene transcription. It localized to both the cytoplasm and the nucleus. It is strongly expressed in heart, brain, placenta, lung, skeletal muscle, kidney, spleen, thymus, prostate, liver, pancreas, testis, ovary, small intestine, colon and peripheral blood leukocytes. Stk40 is able to activate the Erk/MAPK pathway and induce extraembryonic-endoderm (ExEn) differentiation in mouse ESCs. Interestingly, cells overexpressing Stk40 exclusively contribute to the ExEn layer of chimeric embryos when injected into host blastocysts. In contrast, deletion of Stk40 in ESCs markedly reduces ExEn differentiation in vitro. It interacts with Rcn2, which also activates Erk1/2 to induce ExEn specification in mouse ESCs. STK40 has a central serine/threonine protein kinase domain and is homologous to TRB-3. Similarly, overexpression of STK40 inhibits NFκB activation triggered by TNF and also inhibits p53-mediated transcription.

References

Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903.
Wiemann S. et al., 2001, Genome Res. 11 (3): 422-35.
Hartley JL. et al., 2001, Genome Res. 10 (11): 1788-95.

Sino Biological Inc. liefert STK40 Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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