SMPDL3A Protein (His Tag)

04.03.2013

Sino Biological Inc. liefert SMPDL3A Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über SMPDL3A Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/SMPDL3A-Protein-g-10538.html

Produkt-Information von SMPDL3A Protein

Synonym	SMPDL3A, ASML3A
Protein Construction	A DNA sequence encoding the human SMPDL3A (Q92484) (Met1-Tyr453) was expressed with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von SMPDL3A Protein

Purity	> 95% as determined by SDS-PAGE	SDS-PAGE: SMPDL3A protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Leu 23
Molecular Mass	The recombinant human SMPDL3A consists of 442 amino acids and predicts a molecular mass of 50.3 KDa. It migrates as an approximately 51 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von SMPDL3A Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von SMPDL3A Protein

Liver X receptors(LXRs) have been extensively studied in both human and rodent cell systems, revealing their potential therapeutic value in the contexts of atherosclerosis and inflammatory diseases. The LXR genome landscape has been investigated in murine macrophages but not in human THP-1 cells, which represent one of the frequently used monocyte/macrophage cell systems to study immune responses. LXRα and LXRβ function as physiological sensors of cholesterol metabolites (oxysterols), regulating key genes involved in cholesterol and lipid metabolism. SMPDL3A gene is a novel liver X receptor (LXR) -regulated gene, with an LXR response element within its promoter. The induction of SMPDL3A is LXR-dependent and is restricted to human blood cells with no induction observed in mouse cellular systems.

References

Wright KO. et al., 2002, J Urol. 168 (6): 2645-9.
Strausberg RL. et al., 2002, Proc Natl Acad Sci. 99 (26): 16899-903.
Mungall AJ. et al., 2003, Nature. 425 (6960): 805-11.