SIGIRR / TIR8 Protein

16.05.2013

Sino Biological Inc. liefert SIGIRR / TIR8 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über SIGIRR / TIR8 Protein lesen Sie bitte: http://www.sinobiological.com/SIGIRR-TIR8-Protein-a-413.html

Produkt-Information von SIGIRR / TIR8 Protein

Synonym	SIGIRR
Protein Construction	A DNA sequence encoding the human SIGIRR (Q6IA17-1) (Met1-His118) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von SIGIRR / TIR8 Protein

Purity	(96.8+1.6)% as determined by SDS-PAGE	SDS-PAGE: SIGIRR protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human SIGIRR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 359 amino acids and has a predicted molecular mass of 39.5 kDa. The apparent molecular mass of the protein is approximately 47-54 and 33 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von SIGIRR / TIR8 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von SIGIRR / TIR8 Protein

SIGIRR (single immunoglobulin IL-1 receptor-related molecule) is a member of the interleukin 1 receptor (IL-1R) superfamily, and was identified in mouse and human. Although SIGIRR shows the typical conserved motifs that characterize the IL-1R and Toll superfamily, it is structurally and functionally distinct from both. SIGIRR has only one Ig domain in its extracellular portion whereas the IL-1R family contains three Ig folds. An unusually long cytoplasmic domain is reminiscent of the structure of drosophila Toll, yet the SIGIRR peptide sequence is more closely related to IL-1RI. SIGIRR is a negative regulator for Toll-IL-1R signaling. It plays a critical role in gut homeostasis, intestinal inflammation, and colitis-associated tumorigenesis by maintaining the microbial tolerance of the colonic epithelium. SIGIRR-deficient colonic epithelial cells displayed commensal bacteria-dependent homeostatic defects, indicating that SIGIRR is critical in controlling the homeostasis and innate immune responses of the colon to enteric microflora. Moreover, SIGIRR-deficient mice developed stronger Th2 immune response and this discovery suggested that SIGIRR is important in the regulation of Th2, possibly through its impact on IL-33-ST2-mediated signaling.

References

Thomassen E. et al., 1999, Cytokine. 11 (6): 389-99.
Xiao H. et al., 2007, Immunity. 26 (4): 461-75.
Bulek K. et al., 2009, J Immunol. 182 (5): 2601-9.