SHH / Sonic hedgehog (aa 1-197) Protein (His Tag)

Produkt-Information von SHH / Sonic hedgehog Protein

SHH / Sonic hedgehog Protein QC Testing

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: SHH / Sonic hedgehog protein
Bio-activity	Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun.237:465. The ED50 for this effect is typically 2-10 μg/mL.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Cys 24
Molecular Mass	The recombinant human SHH (aa 1-197) consists of 185 amino acids and has a predicted molecular mass of 21 kDa. The apparent molecular mass of the protein is approximately 24 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from a 0.2μm filtered solution of PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von SHH / Sonic hedgehog Protein

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Signal Transduction>>Transcription Factor & Regulator>>SHH / Sonic hedgehog

Beschreibung von SHH / Sonic hedgehog Protein

Sonic HedgeHog, also known as sonic hedgehog protein, belongs to the hedgehog family and is expressed in fetal intestine, liver, lung, and kidney. It cannot be detected in adult tissues. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog exhibits both floor plate- and motor neuron-inducing activity. Defects in sonic hedgehog can cause microphthalmia isolated with coloboma type 5, triphalangeal thumb-polysyndactyly syndrome and holoprosencephaly type.

References

1. Ericson J. et al., 1997, Cold Spring Harb Symp Quant Biol. 62: 451-66.
2. Marigo V. et al., 1996, Proc Natl Acad Sci. 93 (18): 9346-51.
3. Stone DM. et al., 1996, Nature. 384: 129-34.

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