SerpinF2 ELISA Pair Set, Human ELISA Pair Set

Fängerantikörper 0.5 mg/mL of rabbit anti-SERPINF2 monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating.

Nachweisantikörper 0.5 mg/mL rabbit anti-SERPINF2 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0. 5 μg/mL in detection antibody diluteion buffer before use.

Standard Each vial contains 160 ng of recombinant SERPINF2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 4 ng/mL is recommended.

The minimum detectable dose of Human SerpinF2 was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

The Human SerpinF2 ELISA Pair Set is for the quantitative determination of Human SerpinF2.

This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for SerpinF2 coated on a 96-well plate. Standards and samples are added to the wells, and any SerpinF2 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-SerpinF2 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of SerpinF2 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Speicherung - Detection Antibody should be protected from prolonged exposure to light. Aliquot the reagents and store at -20℃ to -70℃ in a manual defrost freezer.

SerpinF2, also known as alpha-2 antiplasmin (alpha-2 AP), is a member of the Serpin superfamily. SerpinF2 is the principal physiological inhibitor of serine protease plasmin, and as well as, an efficient inhibitor of trypsin and chymotrypsin. This protease is produced mainly by liver and kidney, and also expressed at moderate level in other tissues such as muscle, intestine, central nervous system and placenta. Alpha-2 AP is an unusual serpin in that it contains extensive N- and C-terminal sequences flanking the serpin domain. The N-terminal sequence is crosslinked to fibrin by factor XIIIa, whereas the C-terminal region mediates the initial interaction with plasmin. Alpha-2 AP plays the dominant role in inhibiting both plasma clot lysis and thrombus lysis, and accordingly, the deficiency of Alpha-2 antiplasmin in humans results in uncontrolled fibrinolysis and a bleeding disorder.