Coagulation Factor VII / FVII ELISA Pair Set, Mouse ELISA Pair Set

Fängerantikörper 0.3 mg/mL of rabbit anti-mouse FⅦ monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating.

Nachweisantikörper 0.5 mg/mL rabbit anti-mouse FⅦ monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25 μg/mL in detection antibody diluteion buffer before use.

Standard Each vial contains 90 ng of recombinant mouse FⅦ. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 1.5 ng/mL is recommended.

The minimum detectable dose of Mouse Coagulation Factor VII / FVII was determined to be approximately 24 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

The Mouse Coagulation Factor VII / FVII ELISA Pair Set is for the quantitative determination of Mouse Coagulation Factor VII / FVII.

This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Coagulation Factor VII / FVII coated on a 96-well plate. Standards and samples are added to the wells, and any Coagulation Factor VII / FVII present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Coagulation Factor VII / FVII monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Coagulation Factor VII / FVII present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Speicherung - Detection Antibody should be protected from prolonged exposure to light. Aliquot the reagents and store at -20℃ to -70℃ in a manual defrost freezer.

Coagulation factor VII, also known as Serum prothrombin conversion accelerator, Factor VII, F7 and FVII, is a member of the peptidase S1 family. Factor VII is one of the central proteins in the coagulation cascade. It is an enzyme of the serine protease class. Factor VII contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one peptidase S1 domain. The main role of factor VII is to initiate the process of coagulation in conjunction with tissue factor (TF). Tissue factor is found on the outside of blood vessels, normally not exposed to the blood stream. The action of the Factor VII is impeded by tissue factor pathway inhibitor (TFPI), which is released almost immediately after initiation of coagulation. Factor VII is vitamin K dependent and is produced in the liver. The use of warfarin or similar anticoagulants impairs its function. Upon vessel injury, tissue factor is exposed to the blood and circulating Factor VII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. The most important substrates for FVIIa-TF are Factor X and Factor IX.