SAE1 Protein (GST Tag)

27.11.2012

Sino Biological Inc. liefert SAE1 Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über SAE1 Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/SAE1-Protein-g-9918.html

Produkt-Information von SAE1 Protein

Synonym	SAE1, AOS1, SUA1, UBLE1A
Protein Construction	A DNA sequence encoding the human SAE1 (Q9UBE0) (Met1-Lys346) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von SAE1 Protein

Purity	> 95% as determined by SDS-PAGE	SDS-PAGE: SAE1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human SAE1 /GST chimera consists of 583 amino acids and has a calculated molecular mass of 66.3 kDa. The recombinant protein migrates as an approximately 61 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris,500mM NaCl, pH 7.4, 10% gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von SAE1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von SAE1 Protein

SAE1 is a member of the ubiquitin-activating E1 family. It functions as a UBLI E1 ligase mediating the ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. SAE1 binds with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins. As a heterodimer, SAE1 acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4.

References

Gong L. et al., 1999, FEBS Lett. 448 (1): 185-9.
Lois LM. et al., 2005, EMBO J. 24 (3): 439-51.
Tatham MH. et al., 2001, J Biol Chem. 276 (38): 35368-74.