RRM1 Protein (GST Tag)

06.03.2013

Sino Biological Inc. liefert RRM1 Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über RRM1 Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/RRM1-Protein-g-10547.html

Produkt-Information von RRM1 Protein

Synonym	RRM1, RR1
Protein Construction	A DNA sequence encoding the human RRM1 (P23921) (Met1-Ser792) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von RRM1 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: RRM1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human RRM1/GST chimera consists of 1029 amino acids and has a calculated molecular mass of 117.9 kDa. The recombinant protein migrates approximately 98 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 8. 0,3mM DTT, 10% glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von RRM1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von RRM1 Protein

RRM1 is a subunit of ribonucleoside-diphosphate reductase which is constituted by two subunits. Ribonucleoside-diphosphate reductase is an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. RRM1 provides the precursors necessary for DNA synthesis. It catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. RRM1 is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. RRM1 may play a role in malignancies and disease that involve this region.

References

Pitterle DM. et al., 1999, Genomics. 27 (2): 280-5.
Parker NJ. et al., 1995, Proc Natl Acad Sci. 99 (26): 16899-903.
Gautam A. et al., 2003, Oncogene. 22 (14): 2135-42.