RPRD1B Protein (His Tag)

27.05.2013

Sino Biological Inc. liefert RPRD1B Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über RPRD1B Protein (His Tag) lesen Sie bitte:http://www.sinobiological.com/RPRD1B-Protein-Antibody-a-6224.html

Produkt-Information von RPRD1B Protein

Synonym	RPRD1B, C20orf77, CREPT
Protein Construction	A DNA sequence encoding the human RPRD1B (AAH33629.1) (Met1-Asp326) was expressed with an N-terminal polyhistidine tag.
Source	Human
Expression Host	Human Cells

QC Testing von RPRD1B Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE: RPRD1B protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human RPRD1B comprises 346 amino acids and has a predicted molecular mass of 39.3 kDa. The apparent molecular mass of the protein is approximately 39 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von RPRD1B Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von RPRD1B Protein

RPRD1B, together with RPRD1A, can accompany RNAP II from promoter regions to 3'-untranslated regions during transcription in vivo, predominantly interact with phosphorylated RNAP II, and can reduce CTD S5- and S7-phosphorylated RNAP II at target gene promoters. RNA polymerase II C-terminal domain (CTD) phosphorylation is important for various transcription-related processes. RPRD1B is a transcriptional regulator which enhances expression of CCND1. It also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A.

References

Ni Z. et al., 2011, Transcription. 2 (5): 237-42.
Kristensen AR. et al., 2012, Nat Methods. 9 (9): 907-9.
Woods NT. et al., 2012, Sci Signal.5 (242): rs6.