RhoA Antikörper (Antigen Affinität reinigt)

13.02.2012

Produkt-Information von RhoA Antikörper

Immunogen	Recombinant Human RhoA protein (Catalog#12441-H07B)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Human RhoA (rh RhoA;Catalog#12441-H07B; P61586-1; Met 1-Leu 193). RhoA specific IgG was purified by Human RhoA affinity chromatography.

RhoA Antikörper Usage Guide

Specificity	Human RhoA
Western blot	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human RHOA in WB. Using a DAB detection system, the detection limit for Human RHOA is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human RHOA. The detection limit for Human RHOA is approximately 0.00975 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von RhoA Antikörper

Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein which belongs to thesmall GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA / ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA / ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 (diaphanous homolog 1 (Drosophila)). RHOA / ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA / ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA / ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP.

References

Kiss C. et al., 1997, Cytogenet Cell Genet. 79 (3-4): 228-30.
Anastasiadis PZ. et al., 2000, Nat Cell Biol. 2 (9): 637-44.
Yiu G. et al.,2006, Nat Rev Neurosci. 7 (8): 617-27.
Wang HR. et al., 2006, Methods Enzymol. 406: 437-47.
Heo J. et al., 2006, Biochemistry. 45 (48): 14481-9.

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