Rat Thrombomodulin / THBD Protein (His Tag)

11.01.2013

Sino Biological Inc. liefert Rat Thrombomodulin / THBD Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat Thrombomodulin / THBD Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/Thrombomodulin-THBD-Protein-g-10329.html

Produkt-Information von Thrombomodulin / THBD Protein

Synonym	THBD
Protein Construction	A DNA sequence encoding the rat THBD(O35370) (Met1-Ser517) was expressed with a polyhistidine tag at the C-terminus.
Source	Rat
Expression Host	Human Cells

QC Testing von Thrombomodulin / THBD Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: THBD protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ile 17
Molecular Mass	The recombinant rat THBD comprises 512 amino acids and predicts a molecular mass of 55.2 kDa. The apparent molecular mass of the recombinant protein is approximately 95 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von Thrombomodulin / THBD Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von Thrombomodulin / THBD Protein

Thrombomodulin, also known as THBD(CD141), is an integral membrane protein which expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. Thrombomodulin reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. Thrombomodulin also regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene have also been reported to be associated with atypical hemolytic-uremic syndrome.

References

Dzionek A. et al., 2002, Hum Immunol. 63 (12): 1133-48.
Dzionek A. et al., 2000, J Immunol. 165 (11): 6037-46.
Wen DZ. et al., 1987, Biochemistry. 26 (14): 4350-7.