Rat GGT1 Protein (His Tag)

11.12.2012

Sino Biological Inc. liefert Rat GGT1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat GGT1 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/GGT1-Protein-g-9985.html

Produkt-Information von GGT1 Protein

Synonym	GGT1, Ggt
Protein Construction	A DNA sequence encoding the rat GGT1 (P07314) (Pro27-Tyr568) was expressed, fused with a polyhistidine tag at the N-terminus.
Source	Rat
Expression Host	Human Cells

QC Testing von GGT1 Protein

Purity	> 70 % as determined by SDS-PAGE	SDS-PAGE: GGT1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant rat GGT1 comprises 562 amino acids and has a predicted molecular mass of 61.1 kDa. The apparent molecular mass of the protein is approximately 54-57 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von GGT1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von GGT1 Protein

GGT1 is a member of the gamma-glutamyltransferase protein family, of which many members have not yet been fully characterized and some of which may represent pseudogenes. GGT1 is composed of a heavy chain and a light chain and can be detected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. It is part of the cell antioxidant defense mechanism. GGT1 catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. It also initiates extracellular glutathione breakdown, provides cells with a local cysteine supply and contributes to maintain intracelular GSH level. Defects in GGT1 can cause glutathionuria, also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease.

References

Bulle F. et al., 1987, Hum Genet. 76 (3) :283-6.
Tate SS. et al., 1988, Arch Biochem Biophys. 262 (2): 397-408.
Tate SS. et al., 1988, Biochem Biophys Res Commun. 154 (3): 1167-73.