Rat FAS / TNFRSF6 / CD95 Protein (Cytokine)

28.03.2013

Sino Biological Inc. liefert Rat FAS / TNFRSF6 / CD95 Protein (Cytokine) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat FAS / TNFRSF6 / CD95 Protein (Cytokine) lesen Sie bitte: http://www.sinobiological.com/CD95-APO-1-TNFRSF6-Protein-Antibody-a-349.html

Produkt-Information von FAS / TNFRSF6 / CD95 Protein

Synonym	FAS, Apt1, Tnfrsf6
Protein Construction	A DNA sequence encoding the rat FAS (NP_631933.2) (Met1-Lys170) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Rat
Expression Host	Human Cells

QC Testing von FAS / TNFRSF6 / CD95 Protein

Purity	(73+25)% as determined by SDS-PAGE	SDS-PAGE: CD95 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gln 22
Molecular Mass	The recombinant rat FAS/Fc is a disulfide-linked homodimer. The reduced monomer comprises 390 amino acids and has a predicted molecular mass of 43.7 kDa. The apparent molecular mass of the protein is approximately 57 and 36 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von FAS / TNFRSF6 / CD95 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von FAS / TNFRSF6 / CD95 Protein

Tumor necrosis factor receptor superfamily member 6, also known as Apoptosis-mediating surface antigen FAS, FASLG receptor, FAS, and CD95, is a member of the tumor necrosis factor (TNF) / nerve growth factor (NGF) receptor superfamily. FAS is highly expressed on the surface of a number of cell types including epithelial cells, hepatocytes, activated mature lymphocytes and other tumor cells. This receptor contains a death domain and has been shown to play a central role in the physiological regulation of programmed cell death (PCD, apoptosis) upon ligation of Fas ligand (Fas-L). This FAS receptor has been reported to be involved in the activation of NF-kappaB, MAPK3 / ERK1, MAPK8 / JNK, and the alternate pathways for CTL-mediated cytotoxicity. Accordingly, FAS is implicated in the pathogenesis of various malignancies and diseases of the immune system. Multiple Fas isoforms are derived from alternative spliced transcript variants, and among which, a soluble form lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoforms.

References

Suda T. et al., 1993, Cell. 75: 1169-78.
Cascino I. et al., 1995, J Immunol. 154: 2706-13.
Brunner T. et al., 1995, Nature. 373: 441-4.
Hirata H. et al., 1998, J Exp Med. 187: 587-600.
Mary M. et al., 2001, J Biol Chem. 276: 4964-71.
Milhas D. et al., 2005, J Biol Chem. 280 :19836-42.

Cytokine