Rat CD23 / FCER2 / FCER2A Protein

09.06.2013

Sino Biological Inc. liefert Rat CD23 / FCER2 / FCER2A Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rat CD23 / FCER2 / FCER2A Protein lesen Sie bitte: http://www.sinobiological.com/CD23-FCER2-Protein-a-2417.html

Produkt-Information von CD23 / FCER2 / FCER2A Protein

Synonym	FCER2, Fcer2a
Protein Construction	A DNA sequence encoding the rat FCER2 (Q6AZ45) (Glu50-Pro331) was expressed with Fc region of human IgG1 at the N-terminus.
Source	Rat
Expression Host	Human Cells

QC Testing CD23 / FCER2 / FCER2A Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE CD23 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Glu
Molecular Mass	The recombinant rat FCER2 comprises 542 amino acids and predicts a molecular mass of 60.2 kDa. The apparent molecular mass of the recombinant protein is approximately 61 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CD23 / FCER2 / FCER2A Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CD23 / FCER2 / FCER2A Protein

Low affinity immunoglobulin epsilon Fc receptor, also known as BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor, CD23 and FCER2, is a secreted and single-pass type II membrane protein which is also exists as a soluble excreted form. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. Unlike many of the antibody receptors, CD23 / FCER2 is a C-type lectin. It is found on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. In flow cytometry, CD23 / FCER2 is helpful in the differentiation of chronic lymphocytic leukemia (CD23-positive) from mantle cell leukemia (CD23-negative). CD23 / FCER2 can also be demonstrated in germinal centre B-cells using immunohistochemistry, but it is not present in the resting cells of the surrounding mantle zone. CD23 / FCER2 has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).

References

Kikutani H. et al., 1986, Cell. 47: 657-65.
Rose K. et al., 1992, Biochem J. 286: 819-24.
Padlan EA. et al., 1993, Receptor. 3: 325-41.
Bajorath J. et al., 1996, Protein Sci. 5: 240-247.
Barna G. et al., 2008, Hematol Oncol. 26 (3): 167-70.