Rabbit IgG-Fc Protein (Native)

28.02.2012

Produkt-Information von IgG Protein

Synonym :	IgG
Protein Construction:	A DNA sequence encoding the rabbit IgG Fc region (P01870) (Ser 101- Lys 323, 185Thr/Ala , Asn 284/Ser) was expressed and purified.
Source:	Rabbit
Expression Host:	Human Cells

IgG Protein QC Testing

Purity:	> 92 % as determined by SDS-PAGE	SDS-PAGE: IgG protein
Endotoxin:	< 1.0 EU per μg of the protein as determined by the LAL method
Stability:	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal:	Glu 99
Molecular Mass:	The recombinant rabbit IgG -Fc consists of 223 amino acids and has a predicted molecular mass of 25 kDa. As a result of glycosylation, the apparent molecular mass of rabbit IgG-Fc is approximately 33 kDa in SDS-PAGE under reducing conditions.
Formulation:	Supplied as a 0.2μm filtered solution of PBS, pH7.4 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

IgG Protein Usage Guide

Storage:	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Verwandte Produkte & Themen von IgG Protein

Verwandte Bereiche:

Proteine:

Antikörper:

Beschreibung von IgG Protein

Immunoglobulin G (IgG) is a member of many immunoglobulins developed and secreted by effective B cells. IgG is an antibody moleculer induced by infection. Immunoglobulins are composed of four peptides chain-two heavy γ chains and two light chains. These two heavy chains are each linked and linked with one light chain through disulfide bonds. Thus the immunoglobulin forms a Y-like formation. There are two antigen binding sites at the top of Y-like fork's two arms. The disulfide bond site of both heavy chains is also pepsin site. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis. The extent of the changes correlates with the disease severity and reverses in remission. It has been elucidated that the alteration in glycosylation associated with rheumatoid arthritis can create a new mode for the interaction of IgG with complement through binding to the collagenous lectin mannose-binding protein (MBP). Rheumatoid arthritis is associated with a marked increase in IgG glycoforms that lack galactose (referred to as G0 glycoforms) in the Fc region of the molecule and that terminate in N-acetyl glucosamine (GlcNAc).

References

Malhotra R. et al., 1995, Nat Med. 1 (3): 237-43.
Tibbling G. et al., 1977, Scand J Clin Lab Invest. 37 (5): 385-90.
Cartron G. et al., 2002, Blood. 99 (3): 754-8.

Sino Biological Inc. liefert Rabbit IgG-Fc Protein (Native) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Rabbit IgG-Fc Protein (Native) lesen Sie bitte: http://www.sinobiological.com/goods.php?id=8331