PTPN2 / PTPT Protein

28.04.2013

Sino Biological Inc. liefert PTPN2 / PTPT Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über PTPN2 / PTPT Protein lesen Sie bitte: http://www.sinobiological.com/PTPN2-TC-PTP-Protein-a-2452.html

Produkt-Information von PTPN2 / PTPT Protein

Synonym :	PTPN2, PTN2, PTPT, TC-PTP, TCELLPTP, TCPTP
Protein Construction:	A DNA sequence encoding the human PTPN2 ( P17706-1) (Met 1-Asn 314) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Source:	Human
Expression Host:	Baculovirus-Insect cells

QC Testing von PTPN2 / PTPT Protein

Purity:	> 95 % as determined by SDS-PAGE	SDS-PAGE: PTPN2 protein
Endotoxin:	< 1.0 EU per μg of the protein as determined by the LAL method
Stability:	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal:	Gly
Molecular Mass:	The secreted recombinant human PTPN2 consists of 316 amino acids and predicts a molecular mass of 36.8 KDa. The apparent molecular mass of the protein is approximately 37 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation:	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von PTPN2 / PTPT Protein

Storage:	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PTPN2 / PTPT Protein

Tyrosine-protein phosphatase non-receptor type 2, also known as T-cell protein-tyrosine phosphatase, PTPN2 and PTPT, is a cytoplasm protein which belongs to the?protein-tyrosine phosphatase family and Non-receptor class 1 subfamily. Members of the protein tyrosine phosphatase ( PTP ) family share a highly conserved catalytic motif, which is essential for the catalytic activity. TC-PTP / PTPN2 is a cytosolic tyrosine phosphatase that functions as a negative regulator of a variety of tyrosine kinases and other signaling proteins. The expression of TC-PTP / PTPN2 plays a role of tumor suppressor and may modulate response to treatment. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. TC-PTP / PTPN2 is an enzyme that is essential for the proper functioning of the immune system and that participates in the control of cell proliferation, and inflammation. TC-PTP / PTPN2 was identified as a negative regulator of NUP214-ABL1 kinase activity.

References

Iversen L.F. et al., 2002, J Biol Chem. 277: 19982-90.
Stenzinger A. et al., 2005, Histochem Cell Biol. 123: 19-28.
Choudhary C., et al., 2009, Science. 325: 834-40.
Hassan SW. et al., 2010, PLoS One. 5 (1): 8868.