CSNK2A2 / CK2A2 Protein

28.04.2013

Sino Biological Inc. liefert CSNK2A2 / CK2A2 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CSNK2A2 / CK2A2 Protein lesen Sie bitte: http://www.sinobiological.com/CSNK2A2-Protein-a-1422.html

Produkt-Information von CSNK2A2 / CK2A2 Protein

Synonym	CSNK2A2, CK2A2, CSNK2A1, FLJ43934
Protein Construction	A DNA sequence encoding the human CSNK2A2 (NP_001887.1) (Met 1-Arg 350) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von CSNK2A2 / CK2A2 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: CSNK2A2 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gly
Molecular Mass	The secreted recombinant human CSNK2A2 consists of 352 amino acids and predicts a molecular mass of 41.4 KDa. The apparent molecular mass of the protein is approximately 39 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, pH 8.5. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CSNK2A2 / CK2A2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CSNK2A2 / CK2A2 Protein

Casein kinase II subunit alpha', also known as CSNK2A2 and CK2A2, is a member of the protein kinase superfamily, Ser/Thr protein kinase family and CK2 subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. CSNK2A2 is a tetramer composed of an alpha chain, an alpha' and two beta chains. It is also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Protein kinase casein kinase II (Ck2) is a cyclic-AMP and calcium-independent serine-threonine kinase that is composed of two catalytic subunits (alpha and alpha') and two regulatory beta-subunits. Ck2 is not a casein kinase in vivo, but over 100 substrates are known. The highly conserved amino acid sequences of its subunits and their broad expression suggest that Ck2 may have a fundamental role in cell function. Ck2 has been implicated in DNA replication, regulation of basal and inducible transcription, translation and control of metabolism. The Ck2alpha and Ck2alpha' isoforms (products of the genes Csnk2a1 and Csnk2a2, respectively) are highly homologous, the reason for their redundancy and evolutionary conservation is unknown. CSNK2A2 may be a candidate gene for these inherited syndromes.

References

Xu X. et al., 1999, Nat Genet. 23 (1):118-21.
Aasland M. et al., 2000, Anim Genet. 31 (2):131-4.
Escalier D. et al., 2003, Mol Reprod Dev. 66 (2):190-201.
Ackermann K. et al., 2005, Mol Cell Biochem. 274 (1-2):91-101.