PTGDS / L-PGDS Protein (His Tag)

12.12.2012

Sino Biological Inc. liefert PTGDS / L-PGDS Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über PTGDS / L-PGDS Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/PTGDS-L-PGDS-Protein-g-9990.html

Produkt-Information von PTGDS / L-PGDS Protein

Synonym	PTGDS, PDS
Protein Construction	A DNA sequence encoding the human PTGDS (P41222) (Met1-Gln190) was expressed with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von PTGDS / L-PGDS Protein

Purity	> 80% as determined by SDS-PAGE	SDS-PAGE: PTGDS protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ala 23
Molecular Mass	The recombinant human PTGDS consists of 179 amino acids and predicts a molecular mass of 20.1 KDa. It migrates as an approximately 28 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von PTGDS / L-PGDS Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PTGDS / L-PGDS Protein

PTGDS, also known as L-PGDS, is a member of the calycin superfamily, lipocalin family. It is abundantly expressed in the heart, brain, and central nervous system(CNS), where it is expressed in tissues of the blood-brain barrier and secreted into the cerebro-spinal fluid. PTGDS is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of PGH2 to PGD2. It is involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. It binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter.

References

Aebersold R. et al., 1993, Theor Electrophor. 3: 229-34.
Oliver K. et al., 2004, Nature. 429: 369-74.
Bonaldo MF. et al., 1997, Genome Res. 6 (9): 791-806.