PNLIP Protein (His Tag)

28.12.2012

Sino Biological Inc. liefert PNLIP Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über PNLIP Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/PNLIP-Protein-g-10257.html

Produkt-Information von PNLIP Protein

Synonym	PNLIP
Protein Construction	A DNA sequence encoding the human PNLIP (P16233) (Met1-Cys465) was expressed with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von PNLIP Protein

Purity	> 95% as determined by SDS-PAGE	SDS-PAGE: PNLIP protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Lys 17
Molecular Mass	The recombinant human PNLIP consists of 460 amino acids and predicts a molecular mass of 51 KDa. It migrates as an approximately 51 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von PNLIP Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PNLIP Protein

PNLIP is an enzyme which belongs to the lipase family. It is essential for the efficient digestion of dietary fats. Secreted from the pancreas, PNLIP is the primary lipase that hydrolyzes dietary fat molecules in the human digestive system, converting triglyceride substrates found in ingested oils to monoglycerides and free fatty acids. The resulting monomers (2 free fatty acids and one 2-monoacylglycerol) are then moved by way of peristalsis along the small intestine to be absorbed into the lymphatic system by a specialized vessel called a lacteal. Bile salts secreted from the liver and stored in gallbladder are released into the duodenum where they coat and emulsify large fat droplets into smaller droplets, thus increasing the overall surface area of the fat, which allows the lipase to break apart the fat more effectively.

References

Hegele RA. et al., 2001, J Hum Genet. 46 (6): 320-4.
Thomas A. et al., 2005, J Biol Chem. 280 (48): 40074-83.
Colin DY. et al., 2008, Biochem Biophys Res Commun. 370 (3): 394-8.