GIF / Gastric intrinsic factor Protein (His Tag)

28.12.2012

Sino Biological Inc. liefert GIF / Gastric intrinsic factor Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über GIF / Gastric intrinsic factor Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/GIF-Gastric-intrinsic-factor-Protein-g-10256.html

Produkt-Information von GIF / Gastric intrinsic factor Protein

Synonym	GIF, IFMH
Protein Construction	A DNA sequence encoding the human GIF (P27352-1) (Met1-Tyr417) was expressed with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von GIF / Gastric intrinsic factor Protein

Purity	> 95% as determined by SDS-PAGE	SDS-PAGE: GIF protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ser 19
Molecular Mass	The recombinant human GIF consists of 410 amino acids and predicts a molecular mass of 44.8 KDa. It migrates as an approximately 49 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von GIF / Gastric intrinsic factor Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von GIF / Gastric intrinsic factor Protein

Gastric intrinsic factor, also known as GIF, is a glycoprotein produced by the parietal cells of the stomach. It is a member of the cobalamin transport protein family. Gastric intrinsic factor is required for adequate absorption of vitamin B12 in the terminal ileum. Vitamin B12 is essential for erythrocyte maturation and mutations in the Intrinsic Factor may lead to congenital pernicious anemia. Vitamin B12 bounds to haptocorrin after entry into the stomach. The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin. In the less acidic environment of the small intestine, B12 can then bind to gastric intrinsic factor. This new complex travels to the ileum, where special epithelial cells endocytose them. Inside the cell, B12 dissociates once again and binds to another protein, transcobalamin II. The new complex can exit the epithelial cells to enter the liver.

References

Gerdin AK. 2010, Acta Opthalmologica. 88: 925-7.
AU - Berlin H. et al., 1968, Acta Medica Scandinavica. 184 (1-6): 247-58.
Hewitt JE. et al., 1991, Genomics. 10 (2): 432-40.