PFK1 / PFKM Protein (GST Tag)

14.03.2013

Sino Biological Inc. liefert PFK1 / PFKM Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über PFK1 / PFKM Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/PFK1-PFKM-Protein-g-10608.html

Produkt-Information von PFK1 / PFKM Protein

Synonym	PFKM, PFKX
Protein Construction	A DNA sequence encoding the human PFKM (P08237-1) (Thr2-Val780) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von PFK1 / PFKM Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: PFK1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human PFKM /GST chimera consists of 1016 amino acids and has a calculated molecular mass of 112.9 kDa. The recombinant protein migrates as an approximately 113 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von PFK1 / PFKM Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PFK1 / PFKM Protein

PFK1, also known as PFKM, is a regulatory glycolytic enzyme. PFK1 converts fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. It is a muscle-type isozyme. There are three phosphofructokinase isozymes in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Mutations in PFK1 gene have been related with glycogen storage disease type VII, also identified as Tarui disease.

References

Sharma PM. et al., 1990, J Biol Chem. 265 (16): 9006-10.
Nakajima H. et al., 1990, J Biol Chem. 265 (16): 9392-5.
Valdez BC. et al., 1989, Gene. 76 (1): 167-9.