PDGFRA / CD140a Antikörper

09.05.2012

PDGFRA / CD140a Antikörper

Immunogen	Recombinant Human PDGFRA / CD140a protein (Catalog#10556-H08H)
Antibody Type	Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
Clone ID	8G3B9
Ig Type
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PDGFRA / CD140a (rh PDGFRA / CD140a; Catalog#10556-H08H; NP_006197.1; Met 1-Glu 524). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Usage Guide von PDGFRA / CD140a Antikörper

Specificity

Human PDGFRA / CD140a

No cross-reactivity in ELISA with

Human PDGFRb

Human cell lysate (293 cell line)

Immunochemistry

IHC-P: 10-30 μg/mL

PDGFRa-placenta immunohistochemistry PDGFRa-liver immunohistochemistry

Immunochemical staining of human PDGFRa in human placenta with mouse monoclonal antibody (20 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast.

Immunochemical staining of human PDGFRa in human liver with mouse monoclonal antibody (20 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to blood vessel endothelium.

PDGFRa-brain immunohistochemistry

Immunochemical staining of human PDGFRa in human brain with mouse monoclonal antibody (20 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to blood vessel endothelium.

Western blot

This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human PDGFRa in WB.5

Direct ELISA

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human PDGFRa.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃.Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von PDGFRA / CD140a Antikörper

Related Areas:

Enzyme>>Protein Kinase>>Receptor Tyrosine Kinase>>PDGFRA/CD140a

Signal Transduction>>Protein Kinase>>Receptor Tyrosine Kinase>>PDGFRA/CD140a

Stem Cell>>Neural Stem Cell (NSC)>>Neural Stem Cell Markers>>PDGFRA/CD140a

Stem Cell>>Neural Stem Cell (NSC)>>Glial Cell Markers>>PDGFRA/CD140a

Cancer>>Growth factor & Receptor>>PDGF & Receptor>>PDGFRA/CD140a

Cancer>>Growth Factor & Receptor>>Receptor Tyrosine Kinase>>PDGFRA/CD140a

Immunology>>Cluster of Differentiation>>Other>>PDGFRA/CD140a

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
PDGFRA/CD140a	Human	PDGFRA/CD140a/Fc Protein, Recombinant	10556-H02H
PDGFRA/CD140a	Human	PDGFRA/CD140a Protein, Recombinant	10556-H08H
PDGFRA/CD140a	Human	PDGFRA/CD140a Protein, Recombinant	10556-HCCH

Antibodies:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human PDGFRA/CD140a	WB, ELISA, IHC-P	PDGFRA / CD140a Antibody	10556-MM02
Human PDGFRA/CD140a	ELISA	Mouse Monoclonal Antibody	10556-MM03
Human PDGFRA/CD140a	IHC-P	PDGFRA / CD140a Antibody	10556-MM06
Human PDGFRA/CD140a	WB	PDGFRA / CD140a Antibody	10556-MM14
Human PDGFRA/CD140a	WB, ELISA	Rabbit Monoclonal Antibody	10556-R065
Human PDGFRA/CD140a	WB, ELISA	Rabbit Polyclonal Antibody	10556-RP01
Human PDGFRA/CD140a	WB, ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	10556-RP02

Hintergrund von PDGFRA / CD140a Antikörper

PDGFRα, also known as CD140a, together with the structurally homolog protein PDGFRβ (CD140b), are cell surface receptors for members of the platelet-derived growth factor family. They are members of the class III subfamily of receptor tyrosine kinase (RTKs) with the similar structure characteristics of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGFRα is expressed in oligodendrocyte progenitor cells and mesothelial cell, and binds all three ligand isoforms PDGF-AA, PDGF-BB and PDGF-AB with high affinity, whereas PDGFRβ dose not bind PDGF-AA. PDGF binding induces the receptor homo- and hetero-dimerization as well as activation of the receptor, and subsequently activates c-Src tyrosine kinase which relays signals to PI 3 kinase and MAPK cascades. PDGFRα thus plays an essential role in regulating proliferation, chemotaxis and migration of mesangial cells. Recent studies have indicated that PDGF Rα acts as a critical mediator of signaling in testis organogenesis and Leydig cell differentiation, and in addition, particularly important for kidney development.

References

Matsui, T. et al., 1989, Science. 243: 800-804.
Yokote, K. et al., 1996, J. Biol. Chem. 271: 5101-5111.
Choudhury, G.G. et al., 2000, Kidney. Int. 57: 908-917.
Brennan, J. et al., 2003, Genes & Dev. 17: 800-810.
Choudhury, G.G. et al., 2006, Cell. Signal. 18: 1854-18.

Sino Biological Inc. liefert PDGFRA / CD140a Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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