P4HB ELISA Pair Set, Human ELISA Pair Set

04.02.2012

Materialien

1. Materialien gebietet

Fängerantikörper 0.2 mg/mL of mouse anti-P4HB monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating.

Nachweisantikörper 0.5 mg/mL mouse anti-P4HB monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody diluteion buffer before use.

Standard Each vial contains 60 ng of recombinant P4HB. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 1 ng/mL is recommended.

2. Sensitivität

The minimum detectable dose of Human P4HB was determined to be approximately 46.88 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

3. Prinzip des Produkts

The Human P4HB ELISA Pair Set is for the quantitative determination of Human P4HB.

This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for P4HB coated on a 96-well plate. Standards and samples are added to the wells, and any P4HB present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-P4HB monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of P4HB present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Speicherung - Detection Antibody should be protected from prolonged exposure to light. Aliquot the reagents and store at -20℃ to -70℃ in a manual defrost freezer.

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Hintergrund

Prolyl 4-hydroxylase belongs to the group of 2-oxoglutarate coupled dioxygenases, and catalyzes the formation of 4-hydroxyproline in collagens by the hydroxylation of proline residues in peptide linkages. The reaction product, the genetically uncoded amino acid 4-hydroxyproline, plays a crucial role in collagens, as the hydroxyl groups of these amino acid residues are essential for the folding of the newly synthesized procollagen polypeptide chains into triple-helical molecules. The active enzyme is a tetramer (α2β2), and the β subunit (P4HB) alnoe has been found to be identical to the enzyme protein disulfide isomerase (PDI). P4HB is multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. Inside the cell, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins at high concentrations, while facilitates aggregation (anti-chaperone activity) at low concentrations. In addition to a subunit of polyl 4-hydroxylase, P4HB also acts as a structure subunit of microsomal triacylglycerol transfer protein MTTP.

Sino Biological Inc. liefert P4HB ELISA Pair Set, Human ELISA Pair Set mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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