Fetuin-A / AHSG ELISA Pair Set, Mouse ELISA Pair Set

Fängerantikörper 0.5 mg/mL of rabbit anti-mouse AHSG monoclonal antibody, Dilute to a working concentration of 1 μg/mL in CBS before coating.

Nachweisantikörper 0.5 mg/mL rabbit anti-mouse AHSG monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody diluteion buffer before use.

Standard Each vial contains 320 ng of recombinant mouse AHSG. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A six-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 10 ng/mL is recommended.

The minimum detectable dose of Mouse Fetuin-A / AHSG was determined to be approximately 312.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

The Mouse Fetuin-A / AHSG ELISA Pair Set is for the quantitative determination of Mouse Fetuin-A / AHSG.

This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Fetuin-A / AHSG coated on a 96-well plate. Standards and samples are added to the wells, and any Fetuin-A / AHSG present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Fetuin-A / AHSG polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Fetuin-A / AHSG present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Speicherung - Detection Antibody should be protected from prolonged exposure to light. Aliquot the reagents and store at -20℃ to -70℃ in a manual defrost freezer.

Mouse alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The existing major form of AHSG molecule is a disukfied-linked two polypeptide chains which are proteolytic processed from a single proprotein chain. AHSG is a secreted and circulates in blood and extracellular fluids. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix. AHSG is involved in several functions, such as endocytosis, brain development and the formation of bone tissue.