NRAS Protein (His Tag)

02.05.2013

Sino Biological Inc. liefert NRAS Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über NRAS Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/NRAS-N-Ras-Protein-Antibody-a-5927.html

Produkt-Information von NRAS Protein

Synonym	NRAS, HRAS1
Protein Construction	A DNA sequence encoding the mature form of human NRAS (P01111) (Met1-Cys186) was expressed with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von NRAS Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: NRAS protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human NRAS consists of 202 amino acids and predicts a molecular mass of 23 KDa. It migrates as an approximately 24 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 0.1M NaCl, 10% Glycerol, pH 7.5. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von NRAS Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von NRAS Protein

NRAS was discovered by researchers at the Institute of Cancer Research, funded by the Cancer Research Campaign (now Cancer Research UK). NRAS gene is a member of the Ras gene family. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. The mammalian ras gene family consists of the harvey and kirsten ras genes (HRAS and KRAS), an inactive pseudogene of each (c-Hras2 and c-Kras1) and the N-ras gene. They differ significantly only in the C-terminal 40 amino acids. These ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. The NRAS gene specifies two main transcripts of 2Kb and 4.3Kb. The difference between the two transcripts is a simple extension through the termination site of the 2Kb transcript. The NRAS gene consists of seven exons (-I, I, II, III, IV, V, VI).

References

Marshall CJ. et al., 1982, Nature. 299 (5879): 171-3.
Hall Alan. et al., 1983, Nature. 303 (5916): 396-400.
McCormick F. 1996, Mol Reprod Dev. 42 (4): 500-6.