NCKIPSD Protein (GST Tag)

18.04.2013

Sino Biological Inc. liefert NCKIPSD Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über NCKIPSD Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/NCKIPSD-SPIN90-Protein-Antibody-a-5863.html

Produkt-Information von NCKIPSD Protein

Synonym	NCKIPSD, AF3P21, SPIN90
Protein Construction	A DNA sequence encoding the mature form of human NCKIPSD (Q9NZQ3-3) (Met1-Thr244) was expressed with the GST tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von NCKIPSD Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: NCKIPSD protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human NCKIPSD consists of 478 amino acids and predicts a molecular mass of 53.2 KDa. It migrates as an approximately 53 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von NCKIPSD Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von NCKIPSD Protein

NCKIPSD is localized exclusively in the cell nucleus. It plays a role in signal transduction, and may function in the maintenance of sarcomeres and in the assembly of myofibrils into sarcomeres. NCKIPSD also plays an important role in stress fiber formation. NCKIPSD gene is involved in therapy-related leukemia by a chromosomal translocation t(3;11)(p21;q23) that involves this gene and the myeloid/lymphoid leukemia gene. Alternative splicing occurs in this locus and two transcript variants encoding distinct isoforms have been identified. NCKIPSD is a SH3 domain protein. Fas ligand is a cytotoxic effector molecule of T and NK cells which is characterized by an intracellular N-terminal polyproline region that serves as a docking site for SH3 and WW domain proteins. Several previously described Fas ligand-interacting SH3 domain proteins turned out to be crucial for the regulation of storage, expression and function of the death factor. Recent observations, however, indicate that Fas ligand is also subject to posttranslational modifications including shedding and intramembrane proteolysis.

References

Satoh S. et al., 2001, J Biol Chem. 276 (42): 39290-4.
De Bernard M. et al., 2000, EMBO J. 19 (1): 48-56.
Sano K. et al., 2000, Blood. 95 (3): 1066-8.
Voss M. et al., 2009, BMC Immunol. 10: 53.

NCKIPSD (SPIN90) related areas, pathways, and other information