N-Cadherin / CD325 / CDH2 Antikörper

Produkt-Information von N-Cadherin / CD325 / CDH2 Antikörper

Usage Guide von N-Cadherin / CD325 / CDH2 Antikörper

Specificity	Human N-Cadherin / CD325 / CDH2
Flow Cytometry	HeLa cells were harvested with (Left) or without (Right) trypsinization [please note, the epitope is sensitive to trypsin] and stained with either purified Rabbit IgG isotype control (dashed line) or Rabbit Anti-Human CD325 antibody [solid line, and Clone R001 (upper), Clone R020 (bottom)] at matching concentrations, then a FITC-conjugated second step antibody. Flow cytometry analysis of N-Cadherin on HeLa cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
Western blot	This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CDH2 in WB.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CDH2.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃.Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von N-Cadherin / CD325 / CDH2 Antikörper

Neuroscience>>Synaptic Protein>>N-Cadherin/CDH2/CD325

Stem Cell>>Mesenchymal Stem Cell (MSC)>>Mesenchymal Stem Cell Marker>>N-Cadherin/CDH2/CD325

Cancer>>Angiogenesis>>Adhesion Molecules in Angiogenesis>>N-Cadherin/CDH2/CD325

Immunology>>Adhesion Molecule>> Cadherin>>N-Cadherin/CDH2/CD325

Immunology>>Cluster of Differentiation>>N-Cadherin/CDH2/CD325

Hintergrund von N-Cadherin / CD325 / CDH2 Antikörper

Cadherins are calcium dependent cell adhesion proteins, and they preferentially interact with themselves in a homophilic manner in connecting cells. Human cadherin 2, type 1, N-cadherin (neuronal) (NCAD), also known as CDH2, is a single-pass tranmembrane protein and a cadherin containing 5 cadherin domains. N-cadherin displays a ubiquitous expression pattern but with different expression levels between endocrine cell types. CDH2 (NCAD) has been shown to play an essential role in normal neuronal development, which is implicated in an array of processes including neuronal differentiation and migration, and axon growth and fasciculation. In addition, N-cadherin expression was upregulated in human HSC during activation in culture, and function or expression blocking of N-cadherin promoted apoptosis. During apoptosis, N-cadherin was cleaved into 20-100 kDa fragments. N-cadherin cleavage occurred at a higher level in glioblastoma cells than in non-neoplastic astrocytes.

References

1. LaMora A. et al., 2009, Neuroscience. 159: 1175-1184.
2. Chauvet N. et al., 2009, J Endocrinol. 202: 375-387.
3. Hartland S.N. et al., 2009, Liver Int. 29: 966-978.
4. Kohutek Z.A. et al., 2009, J Neurosci. 29: 4605-4615.

Sino Biological Inc. liefert N-Cadherin / CD325 / CDH2 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über N-Cadherin / CD325 / CDH2 Antikörper lesen Sie bitte: http://www.sinobiological.com/N-Cadherin-CD325-CDH2-Antibody-g-8814.html