Mouse SPG21 / ACP33 Protein (GST Tag)

13.04.2012

Produkt-Information von SPG21 / ACP33 Protein

Synonym	ACP33, BM-019, C78576, D9Wsu18e, GL010, MAST
Protein Construction	A DNA sequence encoding the mouse SPG21 isoform 1(Q9CQC8-1) (Met 1-Pro 308) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Mouse
Expression Host	Baculovirus-Insect cells

QC Testing von SPG21 / ACP33 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: SPG21 / ACP33 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The secreted recombinant mouse SPG21/GST chimera consists of 545 amino acids and has a calculated molecular mass of 62.8kDa. The recombinant protein migrates as an approximately 52 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 20mM Tris,500mM NaCl, pH 7.4, 3mM DTT, 10%glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von SPG21 / ACP33 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von SPG21 / ACP33 Protein

Spastic paraplegia 21 (SPG21), also known as Acid Cluster Protein 33 (ACP33) and Mast syndrome protein, is a member of the AB hydrolase superfamily. Human SPG21 is a 308 amino acid residue protein widely expressed in all tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. SPG21 binds to the hydrophobic C-terminal amino acids of CD4 which are involved in repression of T cell activation via the noncatalytic alpha/beta hydrolase fold domain. SPG21 thus is proposed to play a role as a negative regulatory factor in CD4-dependent T-cell activation of CD4. Defects in SPG21 are the cause of spastic paraplegia autosomal recessive type 21, also known as Mast syndrome, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. SPG21 is also associated with dementia and other central nervous system abnormalities.

References

Zeitlmann L. et al., 2001, J Biol Chem. 276: 9123-32.
Simpson M. A. et al., 2003, Am J Hum Genet. 73: 1147-156.
Ota T. et al., 2004, Nat. Genet.36: 40-45.
Kedmi M. et al., 2007, Physiol Genomics. 28: 213-22.
Hanna M. C. et al., 2009, Neurogenetics.10: 217-28.

Sino Biological Inc. liefert Mouse SPG21 / ACP33 Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse SPG21 / ACP33 Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/SPG21-ACP33-Protein-g-8730.html

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat No
SPG21/ACP33	Human	SPG21/ACP33/GST Protein, Recombinant	10522-H09B