Mouse PTK6 / BRK Protein (GST Tag)

18.03.2013

Sino Biological Inc. liefert Mouse PTK6 / BRK Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse PTK6 / BRK Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/PTK6-Brk-Protein-a-739.html

Produkt-Information von PTK6 / BRK Protein

Synonym	PTK6, Sik
Protein Construction	A DNA sequence encoding the mouse PTK6 (Q64434) (Met1-Val451) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Mouse
Expression Host	Baculovirus-Insect cells

QC TestingPTK6 / BRK Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: PTK6 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant mouse PTK6/GST chimera consists of 688 amino acids and has a calculated molecular mass of 79.8 kDa. The recombinant protein migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von PTK6 / BRK Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PTK6 / BRK Protein

Tyrosine-protein kinase 6 (PTK6), also known as Breast tumor Kinase (BRK), is a Src-like nonreceptor tyrosine kinase, which belongs to Tyr protein kinase family. It is overexpressed in the majority of breast cancer and several other cancer types, while being detected at appreciable levels in only a limited range of adult tissues that does not include the mammary gland. PTK6 contains SH3, SH2, and Kinase domains, with a linker region (Linker) between the SH2 and Kinase domains, and the structural basis of the SH3-Linker interaction that results in auto-inhibition of PTK6. Human PTK6 is 79% aa identical to mouse PTK6. PTK6 has been shown to interact with STAP2 and KHDRBS1. In ErbB-Brk-Rac-p38 MAPK pathway, PTK6 is a critical mediator of breast cancer cell migration. The effect of PTK6 on the regulation of phosphoinositide 3-kinase and Akt activity may account for PTK6's ability to enhance mammary cell mitogenesis, raises the possibility that breast tumours. PTK6 is implicated in EGF receptor-dependent signalling and epithelial tumorigenesis. As a stress-induced kinase, it promotes apoptosis by inhibiting prosurvival signaling. In addition, PTK6 may play a key role in lymphomagenesis, hence identifying the kinase as a potential therapeutic target in lymphomas.

References

Chen HY. et al., 2004, Mol Cell Biol. 24 (24): 10558-72.
Haegebarth A. et al., 2006, Mol Cell Biol. 26 (13): 4949-57.
Kasprzycka M. et al., 2006, Am J Pathol. 168 (5): 1631-41.
Ostrander JH. et al., 2007, Cancer Res. 67 (9): 4199-209.
Shen CH. et al., 2008, Cancer Res. 68 (19): 7779-87.
Aubele M. et al., 2008, Br J Cancer. 99 (7): 1089-95.