Mouse AKT3 (aa 106-479) Protein (GST Tag)

18.03.2013

Sino Biological Inc. liefert Mouse AKT3 (aa 106-479) Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse AKT3 (aa 106-479) Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/AKT3-Protein-a-711.html

Produkt-Information von AKT3 Protein

Synonym	AKT3
Protein Construction	A DNA sequence encoding the mouse AKT3 (Q9WUA6-1) (Ala106-Glu479) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Mouse
Expression Host	Baculovirus-Insect cells

QC Testing von AKT3 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: AKT3 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant mouse AKT3/GST chimera consists of 611 amino acids and has a calculated molecular mass of 71 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4, 10% glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von AKT3 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von AKT3 Protein

V-akt murine thymoma viral oncogene homolog 3, also known as AKT3 and protein kinase B gamma (PKBγ), is a member of the AKT, also called PKB, serine/threonine protein kinase family. The AKT family also includes AKT 1 (PKBα) and AKT 2 (PKBβ) and these highly homologous kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. AKT3 has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. AKT3 contains 1 AGC-kinase C-terminal domain, 1 PH domain and 1 protein kinase domain, and binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI3K) results in this cytoplasmic protein kinase targeting to the plasma membrane. In adult tissues, It is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver, whereas in fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney. Alternatively splice transcript variants encoding distinct isoforms have been described.

References

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Walker KS. et al.,1998, Biochem J. 331 (1): 299-308.
Sandirasegarane L. et al., 2001, Biochem Biophys Res. 283 (1): 158-63.
Hodgkinson CP. et al., 2002, Biochemistry. 41 (32): 10351-9.