Mouse NEK3 Protein (GST Tag)

20.04.2012

Produkt-Information von NEK3 Protein

Synonym	NEK3
Protein Construction	A DNA sequence encoding the mouse NEK3 (ABK42288.1 ) (Met 1-Ala 509) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Mouse
Expression Host	Baculovirus-Insect cells

QC Testing von NEK3 Protein

Purity	> 82 % as determined by SDS-PAGE	SDS-PAGE: NEK3 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The secreted recombinant mouse NEK3/GST chimera consists of 746 amino acids and has a calculated molecular mass of 84.8 kDa. The recombinant protein migrates as an approximately 72 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 20mM Tris,500mM NaCl, pH 8.0，10%glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von NEK3 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von NEK3 Protein

NEK3 (NIMA (never in mitosis gene a)-related expressed kinase 3), belongs to the NEK family of protein kinases that share high amino acid homology with NIMA (never in mitosis gene a). NEK3 influences neuronal morphogenesis and polarity through effects on microtubules. It regulates microtubule acetylation in neurons. NEK3 is implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2. NEK3 is up-regulated in malignant versus normal breast tissue. Overexpression of NEK3 in Chinese hamster ovary cells increases cytoskeletal re-organization in response to Prolactin while downregulation of NEK3 expression by siRNA block these effects. Prolactin also stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens.

References

Miller SL. et al., 2007, Oncogene. 26 (32): 4668-78.
Hernández M. et al., 2006, Cancer Invest. 24 (7): 682-8.
Tanaka K. et al., 1999, J Biol Chem. 274 (19): 13491-7.

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