Mouse ITK (aa 351-619) Protein (GST Tag)

08.06.2012

Produkt-Information von ITK / EMT Protein

Synonym	RP23-273O7.1, Emt, Tcsk, Tsk
Protein Construction	A DNA sequence encoding the mouse ITK / EMT (Q03526) protein kinase domain (Arg 351-Leu 619) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Mouse
Expression Host	Baculovirus-Insect cells

QC Testing von ITK / EMT Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: ITK / EMT protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The secreted recombinant mouse ITK /GST chimera consists of 506 amino acids and has a calculated molecular mass of 58.4 kDa. The recombinant protein migrates as an approximately 58 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of 50mM Tris,100mM NaCl,pH 7.5, 10%gly, 0.5mM GSH. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von ITK / EMT Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ITK / EMT Protein

IL-2-inducible T cell kinase contains 1 Btk-type zinc finger, 1 PH domain, 1 protein kinase domain, 1 SH2 domain and 1 SH3 domain. It belongs to the protein kinase superfamily, Tyr protein kinase family and TEC subfamily. IL-2-inducible T cell kinase is an intracellular kinase which expressed in T-cells. It contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. IL-2-inducible T cell kinase also plays an essential role in regulation of the adaptive immune response. It regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. Defects in IL-2-inducible T cell kinase are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1). LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.

References

Lee SH. et al., 2011, Ann Hum Genet. 75 (3): 359-69.
Yao HL. et al., 2010, Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 24 (5): 358-61.
Pechloff K. et al., 2010, J Exp Med. 207 (5): 1031-44.

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