Mouse HGF / Hepatocyte Growth Factor Protein (His Tag)

03.01.2013

Sino Biological Inc. liefert Mouse HGF / Hepatocyte Growth Factor Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse HGF / Hepatocyte Growth Factor Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/HGF-Hepatocyte-Growth-Factor-Protein-g-10266.html

Produkt-Information von HGF / Hepatocyte Growth Factor Protein

Synonym	HGF
Protein Construction	A DNA sequence encoding the mouse HGF(Q08048-1)(Met1-Leu728) was expressed with a C-terminal polyhistidine tag.
Source	Mouse
Expression Host	Human Cells

QC Testing von HGF / Hepatocyte Growth Factor Protein

Purity	> 70% as determined by SDS-PAGE	SDS-PAGE: HGF protein
Bio-activity	Measured in a cell proliferation assay using Mv-1-Lu. The ED50 for this effect is typically 0.8-4 ng/mL.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gln 33
Molecular Mass	The recombinant mouse HGF comprises 707 amino acids and has a predicted molecular mass of 80.8 kDa. The apparent molecular mass of the protein is approximately 92 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von HGF / Hepatocyte Growth Factor Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von HGF / Hepatocyte Growth Factor Protein

Hepatocyte growth factor (HGF), also known as Scatter factor, is a paracrine cellular growth, motility and morphogenic factor. Hepatocyte Growth Factor/HGF is a member of the peptidase S1 family, and is derived from a single chain precursor of 728 amino acid residues containing 4 kringle domains, 1PAN domain and 1peptidase S1 domain. The synthesized single chain of HGF is further processed into a bioactive heterodimer composed of the 69 kDa α-subunit and the 34 kDa β-subunit by a serine protease. Hepatocyte Growth Factor/HGF has no detectable protease activity. Alternative splicing of its gene produces multiple transcript variants encoding different isoforms. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, as well as haemopoietic progenitor cells. Hepatocyte Growth Factor/HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the c-Met receptor. Hepatocyte Growth Factor/HGF may also play an important role as a paracrine or endocrine mediator through an epithelial-mesenchymal interaction in wound-healing, tissue or organ regeneration, morphogenesis and carcinogenesis.

References

Naldini L. et al.,1991, EMBO J. (ENGLAND) 10 (10): 2867-78.
Nakamura T. et al., 1992, Prog Growth Factor Res. 3 (1): 67-85.
Comoglio PM. et al., 1993, EXS. (SWITZERLAND) 65: 131-65.
Skibinski G. et al., 2004, Arch Immunol Ther Exp. 51 (5): 277-82.
Kemp LE. et al., 2006, Biochem Soc Trans. 34 (3): 414-7.