Mouse CD39 / ENTPD1 Protein (His Tag)

07.05.2012

Produkt-Information von CD39 / ENTPD1 Protein

Synonym	2610206B08Rik, AA408691, Cd39, NTPDase-1
Protein Construction	A DNA sequence encoding the mouse ENTPD1 (P55772) extracellular domain (Thr 38-Ile 478) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
Source	Mouse
Expression Host	Baculovirus-Insect cells

QC Testing von CD39 / ENTPD1 Protein

Purity	> 97 % as determined by SDS-PAGE	SDS-PAGE: CD39 / ENTPD1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Thr 38
Molecular Mass	The secreted recombinant mouse ENTPD1 consists of 451 amino acids and has a calculated molecular mass of 51 kDa. The recombinant protein migrates as an approximately 105 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 20mM Tris,500mM NaCl, pH 7.4,10% gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von CD39 / ENTPD1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CD39 / ENTPD1 Protein

CD39, also known as ENTPD1, is a member of the GDA1/CD39 NTPase family. CD39 is weakly expressed on granulocytes while expressed highly on activated lymphoid cells. Human CD39 is found on most mature B cells, activated NK cells and activated T cells. In the nervous system, CD39 could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It could also be implicated in the prevention of platelet aggregation and hydrolyzes ATP and ADP equally well. By hydrolyzing ATP and ADP, CD39 regulates vascular inflammation and thrombosis. It is the dominant ecto nucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type 2 purinergic (P2) G protein coupled receptors (GPCRs). CD39 transgenic mice exhibit impaired platelet aggregation, prolonged bleeding times, and resistance to systemic thromboembolism.

References

Künzli BM. et al., 2011, Dig Dis Sci, 2011 56 (5): 1393-403.
Clayton A. et al., 2011, J Immunol, 187 (2): 676-83.
Loza MJ. et al., 2011, Cell Immunol, 271 (1): 110-7.

Sino Biological Inc. liefert Mouse CD39 / ENTPD1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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