Mouse ART4 / CD297 Protein (His Tag)

31.03.2012

Produkt-Information von ART4 / CD297 Protein

Synonym	4432404K01Rik, DO, DOK1
Protein Construction	A DNA sequence encoding the mature form of mouse ART4 (EDL10575.1) (Met1-Lys 263) was expressed, with a C-terminal polyhistidine tag.
Source	Mouse
Expression Host	Human Cells

QC Testing von ART4 / CD297 Protein

Purity	> 92 % as determined by SDS-PAGE	SDS-PAGE: ART4 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gly 24
Molecular Mass	The recombinant mouse ART4 consists of 251 amino acids and predicts a molecular mass of 28.2 KDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmART4 is approximately 40-45 KDa as a result of glycosylation.
Formulation	Lyophilized from 0.2μm filtered solution of Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

ART4 / CD297 Protein Usage Guide

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ART4 / CD297 Protein

ADP-ribosyltransferase 4 (Dombrock blood group), also known as Mono-ADP-ribosyltransferase 4(ART4), Dombrock blood group carrier molecule and CD297, belongs to a family of ectoenzymes named toxin-related ADP-ribosyltransferases (ARTs) that catalyze the transfer of ADP-ribose from NAD+ to a target protein. ART4 contains a mono-ADP-ribosylation motif. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. ART4 could be detected on HEL cells and erythrocytes by FACS analysis while it was absent from activated monocytes, despite the presence of ART4 mRNA in these cells. ART is also known as the carrier of the Dombrock blood group alloantigens (Do) which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane.

References

Parusel I. et al., 2005, Cell Immunol. 236 (1-2): 59-65.
Friedrich M. et al., 2005, DNA Seq. 16 (1): 53-7.
Okazaki IJ. et al., 1998, J Biol Chem. 273 (37): 23617-20.

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