Micromonospora viridifaciens Neuraminidase / Sialidase Antikörper (Antigen Affinität reinigt)

27.02.2012

Produkt-Information von Micromonospora viridifaciens Neuraminidase / Sialidase Antikörper

Immunogen	Recombinant Micromonospora viridifaciens Neuraminidase / Sialidase protein (Catalog#11679-V07E)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Micromonospora viridifaciens Neuraminidase / Sialidase (Catalog#11679-V07E; BAA00852.1; Pro 42-Gly 403). Micromonospora viridifaciens Neuraminidase / Sialidase specific IgG was purified by Micromonospora viridifaciens Neuraminidase / Sialidase affinity chromatography.

Micromonospora viridifaciens Neuraminidase / Sialidase Antikörper Usage Guide

Specificity	Micromonospora viridifaciens Neuraminidase / Sialidase
Western blot	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Micromonospora viridifaciens Neuraminidase in WB. Using a DAB detection system, the detection limit for Micromonospora viridifaciens Neuraminidase is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Micromonospora viridifaciens Neuraminidase. The detection limit for Micromonospora viridifaciens Neuraminidase is approximately 0.00245 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von Micromonospora viridifaciens Neuraminidase / Sialidase Antikörper

Micromonospora is a genus of bacteria of the family Micromonosporaceae. Micromonospora viridifaciens is one of the Micromonospora species. They are gram-positive, spore-forming, generally aerobic, and form a branched mycelium. They occur as saprotrophic forms in soil and water. NA, also called sialidases, specifically catalyze the hydrolysis removal of terminal sialic acid residues from viral and cellular glycoconjugates. M.viridifaciens neuraminidase catalyzes the hydrolysis of alpha- (2->3)-, alpha- (2->6)-, alpha- (2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates. It belongs to the glycosyl hydrolase 33 family, and contains 5 BRN repeats and 1 F5/8 type C domain. The function of the neuraminidase is to release sialic acids for use as carbon and energy sources for this non-pathogenic bacterium, while in pathogenic microorganisms, sialidases have been suggested to be pathogenic factors.

References

Sakurada, K. et al., 1992, J. Bacteriol. 174: 6896-6903.
Gaskell, A. et al., 1995, Structure. 3: 1197-1205.
Aisaka, K. & Uwajima, T. (1987) FEBS Microbiol. Lett. 44: 289.

Sino Biological Inc. liefert Micromonospora viridifaciens Neuraminidase / Sialidase Antikörper (Antigen Affinität reinigt) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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