MAPKAPK5 Protein (GST Tag)

07.05.2012

Produkt-Information von MAPKAPK5 Protein

Synonym	MAPKAP-K5, MK-5, MK5, PRAK
Protein Construction	A DNA sequence encoding the human MAPKAPK5 isoform 1 (NP_003659.2) (Met 1-Gln 471) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von MAPKAPK5 Protein

Purity	> 76 % as determined by SDS-PAGE	SDS-PAGE: MAPKAPK5 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human MAPKAPK5/GST chimera consists of 708 amino acids and has a calculated molecular mass of 82 KDa. It migrates as an approximately 75KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 20mM Tris,500mM NaCl, pH 8.0, 10%glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von MAPKAPK5 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von MAPKAPK5 Protein

MAPKAPK5 contains 1 protein kinase domain and is a member of the protein kinase superfamily, CAMK Ser/Thr protein kinase family. In response to cellular stress and proinflammatory cytokines, MAPKAPK5 is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, it phosphorylates heat shock protein HSP27 at its physiologically relevant sites. MAPKAPK5 is a t umor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. It mediates stress-induced small heat shock protein 27 phosphorylation. MAPKAPK5 also phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. As a tumor suppressor, it mediates Ras-induced senescence and phosphorylating p53/TP53. It also mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.

References

Ni H. et al., 1998, Biochem Biophys Res Commun. 243 (2): 492-6.
Sudo T. et al., 2000, Biochem Biophys Res Commun. 269 (2): 521-5.
Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26):16899-903.
New L. et al., 2004, Mol Biol Cell. 14 (6): 2603-16.

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