MAP2K6 / MKK6 / MEK6 Protein (GST Tag)

11.04.2012

Produkt-Information von MAP2K6 / MKK6 / MEK6 Protein

Synonym	MAP2K6 ，MAPKK6, MEK6, MKK6, PRKMK6, SAPKK3
Protein Construction	A DNA sequence encoding the human MAP2K6 isoform 1 (P52564-1) (Met 1-Asp 334, Ser 207/Asp, Thr 211/Asp) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von MAP2K6 / MKK6 / MEK6 Protein

Purity	> 88 % as determined by SDS-PAGE	SDS-PAGE: MAP2K6 / MKK6 / MEK6 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human MAP2K6(S207D&T211D）/GST chimera consists of 571 amino acids and has a calculated molecular mass of 65.3 KDa. It migrates as an approximately 60 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 20mM Tris,500mM NaCl, pH 8.0，10%glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von MAP2K6 / MKK6 / MEK6 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von MAP2K6 / MKK6 / MEK6 Protein

Dual specificity mitogen-activated protein kinase kinase 6, also known as MAP kinase kinase 6, MAPKK 6, MAPK / ERK kinase 6, SAPKK3, MAP2K6 and MKK6, is a protein which belongs to the?protein kinase superfamily, STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one?protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors, cytokines, ultraviolet light, and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform?2?of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform?1?of MAP2K6 / MKK6 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.

References

Stein B. et al., 1996, J Biol Chem. 271: 11427-33.
Han J. et al., 1996, J Biol Chem. 271: 2886-91.
Moriguchi T. et al., 1996, J Biol Chem. 271: 13675-9.
Oppermann FS. et al., 2009, Mol Cell Proteomics . 8: 1751-64.
Choudhary C.?et al., 2009, Science. 325: 834-40.

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Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat No
MAP2K6/MKK6/MEK6	Human	MAP2K6 / MKK6 / MEK6 Protein, Recombinant, with GST Tag	10422-H20B