LIF Protein (His Tag)

20.05.2013

Sino Biological Inc. liefert LIF Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über LIF Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/LIF-Protein-a-2625.html

Produkt-Information von LIF Protein

Synonym	LIF, HILDA
Protein Construction	A DNA sequence encoding the human LIF (P15018) (Met1-Phe202) with a C-terminal polyhistidine tag was expressed.
Source	Human
Expression Host	Human Cells

QC Testing von LIF Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: LIF protein
Bio-activity	Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.2-1 ng/ml.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ser 23
Molecular Mass	The recombinant human LIF comprises 191 amino acids and has a predicted molecular mass of 21.2 kDa. The apparent molecular mass of the protein is approximately 35-42 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von LIF Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von LIF Protein

Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It shows a wide range of biologic activities that include the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. LIF has potent proinflammatory property, being the inducer of the acute phase protein synthesis and affecting the cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic and neuronal cells. Due to its polyfunctional activities, LIF is involved in the pathogenic events and development of many diseases of various origin. LIF binds to the specific LIF receptor (LIFR-α) which forms a heterodimer with a specific subunit common to all members of that family of receptors, the GP130 signal transducing subunit. This leads to activation of the JAK/STAT and MAPK cascades. LIF is considered as a direct transcriptional target of STAT5 in myeloid cell lines: STAT5 binds to LIF promoter, and LIF expression is increased after activation of the JAK2/STAT5 pathway. LIF expression is significantly increased in patients with chronic myeloproliferative neoplasms with and without activating mutations of the pathway, indicating that LIF might play an important role in STAT5-mediated oncogenesis.

References

Salas EM. et al. (2011) Genes Cancer. 2 (5): 593-6.
Chodorowska G. et al. (2004) Ann Univ Mariae Curie Sklodowska Med. 59 (2): 189-93.
García-Campaa AM. et al. (2007) Electrophoresis. 28 (1-2): 208-32.