KDM1 / LSD1 Protein (GST Tag)

26.11.2012

Sino Biological Inc. liefert KDM1 / LSD1 Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über KDM1 / LSD1 Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/KDM1-LSD1-Protein-g-9912.html

Produkt-Information von KDM1 / LSD1 Protein

Synonym	KDM1, AOF2, KDM1A, KIAA0601, LSD1
Protein Construction	A DNA sequence encoding the human KDM1 (O60341-1) (Ser172-Met852) was fused with the N-terminal poly histidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von KDM1 / LSD1 Protein

Purity	> 90% as determined by SDS-PAGE	SDS-PAGE: KDM1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human KDM1 /GST chimera consists of 917 amino acids and has a calculated molecular mass of 103.3 kDa. The recombinant protein migrates as an approximately 93 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris,500mM NaCl, 2mM DTT, pH 8.0, 10% gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von KDM1 / LSD1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von KDM1 / LSD1 Protein

LSD1 is a member of the flavin monoamine oxidase family. It acts as a corepressor. It demethylates both mono- and di-methylated 'Lys-4' of histone H3. It contains 1 SWIRM domain and is a component of a RCOR/GFI/LSD1/HDAC complex. LSD1 interacts directly with GFI1 and GFI1B. LSD1 may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. LSD1 demethylates di- methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation.

References

Kusaba M. et al., 2007, 19 (4): 1362-75.
Pazour GJ. et al., 2005, J Cell Biol. 170 (1): 103-13.
Merchant SS. et al., 2007, Science. 318 (5848): 245-50.