JAM-3 / JAM-C Antikörper (Antigen Affinität reinigt)

07.03.2012

Produkt-Information von JAM-3 / JAM-C Antikörper

Immunogen	Recombinant Mouse JAM-3 / JAM-C protein (Catalog#50465-M08H)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Mouse JAM-3 / JAM-C (rM JAM-3 / JAM-C; Catalog#50465-M08H; NP_075766.1; Met 1-Asn 241). JAM-3 / JAM-C specific IgG was purified by Mouse JAM-3 / JAM-C affinity chromatography.

JAM-3 / JAM-C Antikörper Usage Guide

Specificity	Mouse JAM-3 / JAM-C
Western blot	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse JAM3 in WB. Using a DAB detection system, the detection limit for Mouse JAM3 is approximately 4 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse JAM3. The detection limit for Mouse JAM3 is approximately 0.00245 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von JAM-3 / JAM-C Antikörper

Junctional adhesion molecule C, also known as Junctional adhesion molecule 3, JAM3, is a single-pass type I membrane protein which belongs to theimmunoglobulin superfamily. JAM3 is widely expressed. The highest expression of JAM3 is in placenta, brain and kidney. JAM3 contains oneIg-like C2-type (immunoglobulin-like) domain and oneIg-like V-type (immunoglobulin-like) domain. At the protein level JAM3 displays 36 and 32% identity to JAM2 and JAM1, respectively. JAM3 participates in cell-cell adhesion distinct from tight junctions. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. JAM3 is localized in the tight junctions between high endothelial cells. Unlike other proteins in the same family, this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions.

References

Suzuki Y, et al.,1997, Gene. 200 (1-2): 149-56.
Arrate,M.P. et al., 2001, J Biol Chem. 276 (49): 45826-32.
Cunningham, SA. et al., 2002, J Biol Chem. 277 (31): 27589-92.
Muller, WA. et al., 2003, Trends Immunol. 24 (6): 327-34.

Sino Biological Inc. liefert JAM-3 / JAM-C Antikörper (Antigen Affinität reinigt) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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