HGF Antikörper (Antigen Affinität reinigt)

07.03.2012

Podukt-Information von HGF Antikörper

Immunogen	Recombinant Human HGF protein (Catalog#10463-HNAC)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Human HGF (rh HGF;Catalog#10463-HNAC; NP_000592.3; Met 1-Ser 728). HGF specific IgG was purified by Human HGF affinity chromatography.

HGF Antikörper Usage Guide

Specificity	Human HGF
Western blot	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human HGF in WB. Using a DAB detection system, the detection limit for Human HGF is approximately 0.5 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human HGF. The detection limit for Human HGF is approximately 0.00245 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von HGF Antikörper

Hepatocyte growth factor (HGF), also known as Scatter factor, is a paracrine cellular growth, motility and morphogenic factor. HGF is a member of the peptidase S1 family, and is derived from a single chain precursor of 728 amino acid residues containing 4 kringle domains, 1PAN domain and 1peptidase S1 domain. The synthesized single chain of HGF is further processed into a bioactive heterodimer composed of the 69 kDa α-subunit and the 34 kDa β-subunit by a serine protease. HGF has no detectable protease activity. Alternative splicing of its gene produces multiple transcript variants encoding different isoforms. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, as well as haemopoietic progenitor cells. HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the c-Met receptor. HGF may also play an important role as a paracrine or endocrine mediator through an epithelial-mesenchymal interaction in wound-healing, tissue or organ regeneration, morphogenesis and carcinogenesis.

References

Naldini, L. et al.,1991, EMBO J. (ENGLAND)10 (10): 2867-78.
Nakamura, T. et al., 1992,Prog. Growth Factor Res.3 (1): 67-85.
Comoglio, PM. et al., 1993, EXS. (SWITZERLAND)65: 131-65.
Skibinski, G. et al., 2004, Arch. Immunol. Ther. Exp. 51 (5): 277-82.
Kemp, LE. et al., 2006, Biochem. Soc. Trans.34 (Pt 3): 414-7.

Sino Biological Inc. liefert HGF Antikörper (Antigen Affinität reinigt) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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