ITCH / AIP4 Antikörper ( FITC )

Sino Biological Inc. liefert ITCH / AIP4 Antikörper ( FITC ) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über ITCH / AIP4 Antikörper ( FITC ) lesen Sie bitte: http://www.sinobiological.com/ITCH-AIP4-Antibody-g-10062.html

Produkt-Information von ITCH / AIP4 Antikörper

Usage Guide von ITCH / AIP4 Antikörper

Specificity	Human ITCH / AIP4
Flow Cytometry	Flow cytometric analysis of Human ITCH expression in HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and then stained with FITC Mouse anti-ITCH (11131-MM01-F). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
Storage	This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Verwandte Produkte & Themen von ITCH / AIP4 Antikörper

Neuroscience>>Neurodegenerative Disease>>Ubiquitin-proteasome pathway>>ITCH/AIP4

Hintergrund von ITCH / AIP4 Antikörper

E3 ubiquitin-protein ligase Itchy homolog, also known as Atrophin-1-interacting protein 4, NFE2-associated polypeptide 1, NAPP1 and ITCH, is a cell membrane protein which contains oneC2 domain, oneHECT (E6AP-type E3 ubiquitin-protein ligase) domain and contains fourWW domains. ITCH acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. ITCH is involved in the control of inflammatory signaling pathways. It is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. ITCH promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteosomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Defects in ITCH are the cause of syndromic multisystem autoimmune disease (SMAD) which is characterized by organomegaly, failure to thrive, developmental delay, dysmorphic features and autoimmune inflammatory cell infiltration of the lungs, liver and gut.

References

1. Marchese A. et al., 2003, Dev. Cell 5:709-22.
2. Wang Y. et al., 2006, EMBO J. 25: 5058-70.
3. Bhandari D. et al., 2009, Mol. Biol. Cell 20: 1324-39.
4. Edwards TL. et al., 2009, Biochem. J. 423: 31-9.
5. Zhang P. et al., 2010, J. Biol. Chem. 285: 8869-79.
6. Azakir B.A. et al., 2010, FEBS J. 277: 1319-30.

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