IL-33 Antikörper

Sino Biological Inc. liefert IL-33 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über IL-33 Antikörper lesen Sie bitte: http://www.sinobiological.com/IL-33-Antibody-g-10075.html

Produkt-Information von IL-33 Antikörper

Usage Guide von IL-33 Antikörper

Specificity	Human IL-33
Flow Cytometry	Flow cytometric analysis of Purified anti-Human IL-33 on HUVEC cells. HUVEC cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Rabbit anti-IL-33 (Bold line hisgram), then stained with a FITC-conjugated second step antibody. Flow cytometry was performed on a BD FACSCalibur flow cytometry system Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human IL33. The detection limit for Human IL33 is approximately 0.00245 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von IL-33 Antikörper

Immunology>>Adaptive Immunity>>T Cell>>Helper T Cell>>IL-33

Immunology>>Cytokine & Receptor>>Interleukin & Receptor >>IL-1 superfamily and IL-1R/TLR family>>IL-33

Hintergrund von IL-33 Antikörper

Interleukin-33 (IL-33), initially discovered as a nuclear factor NF-HEV abundantly expressed in high endothelial venules, also known as DVS 27, is a proinflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyers's patches, and lymph nodes. It contains a bipartite nuclear localization signal at the C-terminus, and is targeted to the nucleus when ectopically expressed in human umbilical vein endothelial cells (HUVECs) and HeLa cells. The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling. IL-33 mediates its biological effects via Toll-interleukin 1 (IL-1) receptor (TIR) domain-containing receptor ST2, activates NF-kappaB and MAP kinases, and drives production of T (H)2-associated cytokines from in vitro polarized T (H)2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs.

References

1. Cayrol C,Girard JP, 2009, Proc Natl Acad Sci U S A. 106 (22): 9021-6.
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3. Baekkevold, E.S. et al., 2003, Am. J. Pathol. 163:69.
4. Schmitz, J. et al., 2005, Immunity. 23:479.
5. Black, R.A. et al., 1989, J. Biol. Chem. 264: 5323.
6. Xu, D. et al., 1998, J. Exp. Med. 187: 787.
7. Dinarello, C.A., 2005, Immunity. 23: 461.

Primary Antibody Products