IgG1 Fc Protein (Native)

24.04.2012

Produkt-Information von IgG1 Protein

Synonym	IgG1
Protein Construction	A DNA sequence encoding the human IgG1 Fc region (AAC82527.1) (Glu 99- Lys 330 ) (one aa mutation,103 Cys/Ser) was expressed.
Source	Human
Expression Host	CHO Cells

QC Testing von IgG1 Protein

Purity	> 92 % as determined by SDS-PAGE	SDS-PAGE: IgG1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Glu 99
Molecular Mass	The recombinant human IgG1 Fc consists of 232 amino acids and has a predicted molecular mass of 26 kDa. As a result of glycosylation, the apparent molecular mass of rhFc is approximately 34 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von IgG1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Verwante Produkte & Themen von IgG1 Protein

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Beschreibung von IgG1 Protein

As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases.

References

Carosella ED. et al., 1988, Cell Immunol. 112: 262-70.
Eckle I. et al., 1990, Clin Exp Immunol. 81: 352-6.
Lorriaine C. et al., 1995, J Biol Chem. 270:8164-71.
Gomez-Guerrero C. et al., 2000, J Immunol. 164: 2092-101.
Sprague ER. et al., 2004, J Biol Chem. 14: 14184-93.
Anthony RM. et al., 2008, Science. 320: 373-6.

Sino Biological Inc. liefert IgG1 Fc Protein (Native) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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