IFI30 Protein (His Tag)

07.09.2012

Produkt-Information von IFI30 Protein

Synonym	IFI30, GILT, IP30
Protein Construction	A DNA sequence encoding the human IFI30 (P13284)(Met1-Lys243 )with a C-terminal polyhistidine tag was expressed.
Source	Human
Expression Host	Human Cells

QC Testing von IFI30 Protein

Purity	> 96 % as determined by SDS-PAGE	SDS-PAGE: IFI30 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ser 27
Molecular Mass	The recombinant human IFI30 is a disulfide-linked homodimer. The reduced monomer comprises 217 amino acids and has a predicted molecular mass of 24.7 kDa. The apparent molecular mass of the protein is approximately 31-34 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von IFI30 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von IFI30 Protein

IFI30 is a member of the GILT family. This family contains proteins homologous to the two characterised human gamma-interferon-inducible lysosomal thiol reductase. It also contains several other eukaryotic putative proteins with similarity to GILT. The aligned region contains three conserved cysteine residues. In addition, the two GILT sequences possess a C-X(2)-C motif that is shared by some of the other sequences in the family. This motif is thought to be associated with disulphide bond reduction. IFI30 is a lysosomal thiol reductase that can reduce protein disulfide bonds. It may facilitate the complete unfolding of proteins destined for lysosomal degradation. IFI30 also facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Meanwhile, it facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation. IFI30 plays an important role in antigen processing.

References

Haque MA. et al., 2002, J Exp Med. 195 (10): 1267-77.
Phan UT. et al., 2000, J Biol Chem. 275 (34): 25907-14.
Phan UT. et al., 2001, Immunogenetics. 53 (4): 342-6.

Sino Biological Inc. liefert IFI30 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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