IDH1 Antikörper

Sino Biological Inc. liefert IDH1 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über IDH1 Antikörper lesen Sie bitte: http://www.sinobiological.com/IDH1-Antibody-g-10057.html

Produkt-Information von IDH1 Antikörper

Usage Guide von IDH1 Antikörper

Specificity	Human IDH1
Flow Cytometry	Flow cytometric analysis of Human IDH1 expression in HepG2 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-IDH1 (12055-MM03), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von IDH1 Antikörper

Enzyme>>Other>>IDH1

Hintergrund von IDH1 Antikörper

Isocitrate dehydrogenases 1 (IDH1), one of the Isocitrate dehydrogenases, which catalyze the oxidative decarboxylation of isocitrate to α-ketoglutarate and reduce NAD (P)+ to NAD (P)H. This process involves oxidation of isocitrate to oxalosuccinate, with NAD (P)H as the electron acceptor, followed by decarboxylation of oxalosuccinate to form α-ketoglutarate. IDH1 and IDH2 function at a crossroads of cellular metabolism in lipid synthesis, cellular defense against oxidative stress, oxidative respiration, and oxygen-sensing signal transduction. IDH1 and IDH2 mutations are usually heterozygous in cancer, and they appear to confer a neomorphic enzyme activity for the enzymes to catalyze the production of D-2-hydroxyglutarate. IDH1 is highly expressed in the mammalian liver and moderately expressed in other tissues. It contains a C-terminal tripeptide peroxisome targeting signal 1 sequence. Isocitrate dehydrogenase 1 (IDH1) mutations have recently been identified as early and frequent genetic alterations in astrocytomas, oligodendrogliomas, and oligoastrocytomas, as well as secondary glioblastomas, whereas primary glioblastomas very rarely contain IDH1 mutations.

References

1. Reitman ZJ. et al., 2010, J Natl Cancer Inst. 102 (13): 932-41.
2. Gabriel JL. et al., 1986, Metabolism. 35 (7): 661-667.
3. Jennings GT. et al., 1994, J Biol Chem. 269 (37): 23128-23134.
4. Takano S. et al.. 2011, Brain Tumor Pathol. 28 (2): 115-23.

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