Gremlin 1 / GREM1 Protein

12.10.2012

Produkt-Information von Gremlin 1 / GREM1 Protein

Synonym	GREM1, Cktsf1b1, Drm
Protein Construction	A DNA sequence encoding the mouse GREM1(O70326) (Met1-Asp184) was fused with a polyhistidine tag at the C-terminus.
Source	Mouse
Expression Host	Baculovirus-Insect cells

QC Testing von Gremlin 1 / GREM1 Protein

Purity	> 97 % as determined by SDS-PAGE	SDS-PAGE: Gremlin 1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Lys 25
Molecular Mass	The recombinant mouse GREM1 consists of 171 amino acids and has a calculated molecular mass of 19.7 kDa. The recombinant protein migrates as an approximately 25 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH7.4,10% gly,0.5mM EDTA, 3mM DTT. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von Gremlin 1 / GREM1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von Gremlin 1 / GREM1 Protein

GREM1 is a member of the DAN family. It contains 1 CTCK (C-terminal cystine knot-like) domain and is highly expressed in small intestine, fetal brain and colon. As a cytokine, GREM1 may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. It interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner and down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis. GREM1 also is an inhibitor in the TGF beta signaling pathway. It inhibits BMP-2, -4, and -7. Inhibition by grem 1 of BMPs in mice allow the expression of fibroblast growth factors (FGFs) 4 and 8 and Sonic hedgehog (SHH) which are necessary for proper limb development.

References

Dimitrov BI. et al., 2010, J Med Genet. 47 (8): 569-74.
Heron M. et al., 2011, Tissue Antigens. 77 (2): 112-7.
Van Vlodrop IJ. et al., 2010, Am J Pathol. 176 (2): 575-84.

Sino Biological Inc. liefert Gremlin 1 / GREM1 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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