GPR114 Protein

05.06.2012

Produkt-Information von GPR114 Protein

Synonym	PGR27, GPR114
Protein Construction	A DNA sequence encoding the human GPR114 (Q8IZF4) (Met1-Gly184) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von GPR114 Protein

Purity	>94 % as determined by SDS-PAGE	SDS-PAGE: GPR114 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Glu 22
Molecular Mass	The recombinant human GPR114/Fc is a disulfide-linked homodimer. The reduced monomer comprises 404 amino acids and has a predicted molecular mass of 45.6 kDa. The apparent molecular mass of the protein is approximately 56-63 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von GPR114 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von GPR114 Protein

GPR114 contains 1 GPS domain and is a member of the G-protein coupled receptor 2 family. G-protein-coupled receptors (GPCRs) share a common molecular architecture which consists of seven transmembrane domains, three extracellular loops, three intracellular loops, an amino-terminal extracellular domain and an intracellular carboxyl terminus. Light is thought to be the activating stimulus of a GPCR. GPCRs are expected to have molecular function (G-protein coupled receptor activity) and to localize in various compartments (endoplasmic reticulum membrane, plasma membrane, integral to membrane). Family B of the GPCRs is a small but structurally and functionally diverse group of proteins that includes receptors for polypeptide hormones, molecules thought to mediate intercellular interactions at the plasma membrane and a group of Drosophila proteins that regulate stress responses and longevity. GPR114 gene has been proposed to participate in processes (G-protein coupled receptor protein signaling pathway, neuropeptide signaling pathway).

References

Ota T. et al., 2004, Nat Genet. 36 (1): 40-5.
Bjarnadóttir TK. et al., 2005, Genomics. 84 (1): 23-33.
Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.

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