GOLM1 / GP73 Antikörper

21.08.2012

Produkt-Information von GOLM1 / GP73 Antikörper

Immunogen	Recombinant Human GOLM1 / GP73 protein (Catalog#13066-H07E)
Antibody Type	Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
Clone ID	06
Ig Type
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human GOLM1 / GP73 (rh GOLM1 / GP73; Catalog#13066-H07E; Q8NBJ4-1; Met 1-Leu 401). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Usage Guide von GOLM1 / GP73 Antikörper

Specificity

Human GOLM1 / GP73

Immunochemistry

IHC-P: 10-30 μg/mL

GP73-liver immunohistochemistry GP73-colon-carcinoma immunohistochemistry

Immunochemical staining of human GP73 in human liver with mouse monoclonal antibody (15 μg/mL, formalin-fixed paraffin embedded sections).

Immunochemical staining of human GP73 in human colon carcinoma with mouse monoclonal antibody (15 μg/mL, formalin-fixed paraffin embedded sections).

GP73-stomach immunohistochemistry

Immunochemical staining of human GP73 in human stomach with mouse monoclonal antibody (15 μg/mL, formalin-fixed paraffin embedded sections).

Western blot

This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human GP73 in WB.

Direct ELISA

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human GP73.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von GOLM1 / GP73 Antikörper

Related Areas:

Cancer >>Cancer Biomarker>>GOLM1

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
GOLM1	Human	GOLM1 Protein, Recombinant	13066-H08H
GOLM1	Human	GOLM1 Protein, Recombinant	13066-H07E

Antibodies:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human GOLM1	ELISA	GOLM1 Antibody, Rabbit Monoclonal	13066-R005
Human GOLM1	ELISA	GOLM1 Antibody, Rabbit Monoclonal	13066-R024
Human GOLM1	WB, FCM	GOLM1 / GP73 Antibody	13066-R024-F
Human GOLM1	WB, ELISA, IHC-P	GOLM1 / GP73 Antibody	13066-R037
Human GOLM1	WB, IHC-P	GOLM1 / GP73 Antibody	13066-R123
Human GOLM1	WB, ELISA, IHC-P	GOLM1 / GP73 Antibody (Antigen Affinity Purified)	13066-RP02
Human GOLM1	WB, ELISA	GOLM1 / GP73 Antibody (Antigen Affinity Purified)	13066-RP04
Human GOLM1	WB, ELISA	GOLM1 / GP73 Antibody	13066-MM01
Human GOLM1	WB, ELISA, IHC-P	GOLM1 / GP73 Antibody	13066-MM06
Human GOLM1	WB, ELISA	GOLM1 / GP73 Antibody	13066-MM15

Hintergrund von GOLM1 / GP73 Antikörper

Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2 and GOLM1, is a protein which belongs to theGOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in colon, prostate, trachea and stomach. It is expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncitial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.

References

Kladney R.D., et al., 2000,Gene 249:53-65.
Kladney R.D., et al., 2002, Virology 301:236-246.
Ramachandran P., et al., 2006, J. Proteome Res. 5:1493-1503.
Zhou W.,et al., 2009, J. Proteome Res. 8:5523-5531.

Sino Biological Inc. liefert GOLM1 / GP73 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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